Production evaluation of Lentinula edodes Pegler fungi in synthetic logs based on agricultural wastes

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Valeska Villegas Escobar
Ana Milena Pérez
Clara Arredondo


Lentinula edodes, shiitake, agroindustrial wastes, medicinal and gourmet mushrooms.


Production of Lentinula edodes, medicinal and gourmet mushroom commonly known as shiitake, was evaluated in synthetic blocks with the aim of using agroindustrial wastes not jet studied quantitatively and environmentally problematical for many industries. Fifty five different combinations of substrates were analyzed using two agroindustrial wastes (cocoa husk and cotton waste), one wood supplement (oak), one nitrogen source (wheat bran), one pH controller (CaCO3), and one growth stimulator (CaSO4). It was found that the substrate formulation has a considerable effect upon the colonization time of the block (P < 0.05), being the oak supplement essential for the time colonization reduction. Furthermore, the cocoa husk was not a good waste for shiitake production. The treatments that gave the best conditions for shiitake culture contained 75% of oak, and 20 to 25% of wheat bran or 25% of cotton waste. Depending on the treatment evaluated, the biological efficiency achieved were between 5.3 to 21.5%, the pileo size from 4.7 to 9.3 cm for the first flush and the colonization precocity between 69 to 125 days with C/N relationship superior to 110 and with better nutritional value to those reported by other researchers.


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