application ZERI methodology in the production of alkaline protease from Bacillus sp.

Abstract

The possibility of producing  culture means from waste, such as water resulting from potato washing (PW) and hydrolyzed dust from leather (HDL), for the production of alkaline proteases from a native culture of Bacillus  sp. Alcalophilic was determined. The growth of the microorganism and the production of the true protein are not affected by the inoculation pH of 7.0, 8.5 and 9.5. This differs to the enzymatic activity that had its best results at pH of 9.5, which  was selected to evaluate two organic nitrogen  sources (HDL and peptone) in PW at different C/N ratios (between 0 and 4). After evaluating different concentration of nitrogen sources, the highest protein production was when C/N is 3, with peptone and concentration  of starch of 0.5%, similar to the maximum value obtained using HDL with 1% of starch and C/N of 1. These similarities allow us to suggest this enzyme for further  studies in the making of culture medias with the goal of reducing environmental impact framed on the concept of ZERI.