Proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico

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A. Sáez Vega


Bacillus, proteases, enzymes.


The effect of four nitrogen sources on the enzymatic activity of alkaline proteases from a wild strain of Alkalophilic Bacillus sp cultivated to different concentrations from CSL (Corn Steep Liquor). was evaluated. The growth of the strain is not affected by pH of inoculation of 7,0, 8,5 and 9,5, in contrast to the enzymatic activity and true protein production, that had their better results to initial pH of 8,5. To this initial pH of 8,5; two organic nitrogen sources (yeast extract and peptone) and two inorganic ones were evaluated (NH4Cl and NaNO3). With peptone the best enzymatic activity to a relation appeared to molar C/N between 1 and 2 was found. For the studied interval of % CSL (0,5 to 2% p/v) the concentration of the CSL does not affect the enzymatic activity.

PACS: 87.17.Uv


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[1] K. Uraguchi y M. Yamazaki. Toxicology, Biochemistry and pathology of Mycology. Ed. Japón, John Wiley & Son, Japón, 1978.

[2] D. Pérez et al. Ciencias Biológicas. 17, Cuba, 1987.

[3] A. Simoncini. Subtilinases production. J. Of American Leather Chemistry Association. 82, 1987.

[4] Knud Aunstrup et al. Alkaline proteases produced by a bacillus. Patente US 03674643, 1972.

[5] T. Christianson et al. Alkaline proteases variants with increases stability. US Patent. 5340735, 1994.

[6] R. Harwood Colin. Bacillus. New York: Plenum Press, ISBN 0-306-43137-8, 1989.

[7] N. Fujiwara y K. Yamamoto Production of alkaline protease in a low-cost medium by Alkalophilic Bacillus sp. J. Ferment. Technol. 63(3), 1987.

[8] J. E. Donnellan. Chemically defined, synthetic media for sporulation and for germination and growth of Bacillus subtilis. J. Bacteriology. 87(2), 1964.

[9] J. L. Doering y K.F. Bott. Diferential aminoacids requirements for sporulations in B. subtilis. J. Bacteriol. 112(1), 1972.

[10] S. Moon y S. Parulekar. A parametric study of a protease production in batch culture of Bacillus firmus. J. Biotech. and Bioeng. 41(1), 1993.

[11] Olga Inés Montoya. Aislamiento de cepas nativas de Bacillus sp. Alcalofílicas y obtención de proteasas alcalinas. Tesis, Universidad Nacional de Colombia, Santa Fé de Bogotá, 1997.

[12] G. Giraldo y A. Gómez. Selección del mejor medio de cultivo para la obtención de proteasas alcalinas de la cepa nativa Bacillus sp. Alcalofílico. Tesis, Universidad de Antioquia, Medellín, 1999.

[13] A. Van Velzen. Determination of alkaline protease activity. US Patent. 1353317, 1976.
[14] O. H. Lowry, A. L. Rosebrough et al. Protein measurement with the Folin phenol, reagent. J. Biol. Chem. 193, 1951.

[15] R. C. Avignone y O. Yantorno. Organic and Inorganic Nitrogen sources ratio effects on Bt. World Journal of Microbiology and Biotechnology. 6, 1990.

[16] Leo Goldblatt. Aflatoxins (Serie: Food science and Technology). New York: Academic Press, 1969.