Following the titration monoprotic acid–strong base with High Performance Liquid Chromatography –HPLC–
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Keywords
titration, chromatographic titration curves, monoprotic organic acids, HPLC.
Abstract
This article proposes the High Performance Liquid Chromatography as an alternative to realize the follow up of the weak acids with strong bases titrations. Taking advantage of the kindness of this powerful instrumental technology it is possible to visualize in real time all the substances involved in the process to quantify with extraordinary precision the variation of its concentration and to generate new graphs that allow analyzing the advance of the neutralization process from different perspectives. It is studied the titration case of the acetic one as weak monoprotic acid by a strong base, sodium hydroxide, applying a specific chromatographic method for organic acids.
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References
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[4] Cristina Rodríguez, José M. Amigo, Jordi Coello y Santiago Maspoch. An In- troduction to Multivariate Curve Resolution-Alternating Least Squares: Spectrophotometric Study of the Acid–Base Equilibria of 8-Hydroxyquinoline-5-sulfonic Acid. Journal of Chemical Education, ISSN 0021-9584, 84(7), 1190–1192 (2007).
[5] Keenan Dungey and Paul Epstein. Titration of a solid acid monitored by X-ray diffraction. Journal of Chemical Education, ISSN 0021–9584, 84(1), 122–123 (2007).
[6] Karen M. Gooding. HPLC of Biological Macromolecules: Methods and Applications, ISBN 978-0824778798, Fred E. Regnier (Editor), 796 (1990).
[7] Douglas A. Skoog y James J. Leary. Análisis Instrumental, ISBN 0–03–023343-7, 4◦ edición. McGraw–Hill, México, 730–777 (1994).
[8] Vera Canelas and Cristina Teixeira da Costa. Quantitative HPLC Analysis of Rosmarinic Acid in Extracts of Melissa officinalis and Spectrophotometric Measurement of Their Antioxidant Activities. Journal of Chemical Education, ISSN 0021–9584, 84(9), 1502–1504 (2007).
[9] W. F. Chan and C. W. Lin. High-Pressure Liquid Chromatography: Quantitative Analysis of Chinese Herbal Medicine. Journal of Chemical Education, ISSN 0021– 9584, 84(12), 1982–1984 (2007).
[10] M. Tormo and J. M. Izco. Alternative reversed-phase high-performance liquid chromatography method to analyse organic acids in dairy products. Journal of Chromatography A, 1033(2), 305–310 (2004).
[11] Oleg Prezhdo, Collen F. Craig, Yuriy Fialkov and Victor Prezhdo. Control of chemical equilibrium by solvent: A basis for teaching physical chemistry of solutions. Journal of Chemical Education, ISSN 0021-9584, 84(8), 1348–1354 (2007).
[12] Judith F. Rubinson y Kenneth A. Rubinson. Química Analítica Contemporánea, ISBN 970–17–03412–1. Prentice Hall, México, 168–190 (2000).
[13] A. Popov and R. Pflaum. Introductory Analytical Chemistry, ISBN 978- 0669252392. DC Heath. Library of Congress Catalog Card Number 66–17008, 298 (1967).
[14] O. Budevsky, R. A. Chalmers and Mary R. Masson. Foundations of Chemical Analysis, ISBN 0–85312–113–3. Ellis Horwood Limited. New York, 44–54 (1979).
[15] Rubén Darío Osorio Giraldo. Métodos numéricos en Química con Matlab, ISBN 978–958–714–044–6. Editorial Universidad de Antioquia, Medellín. 127– 139 (2007).